The rumours began when the singer appeared to have a ‘bulge’ on stage at Glastonbury in June.
A false quote attributed to Lady Gaga, 23, was also circulated on the internet.
‘It’s not something that I’m ashamed of,’ she was reported to have said. ‘It just isn’t something that I go around telling everyone.
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‘I have both male and female genitalia, but I consider myself a female. It’s just a little bit of a penis and really doesn’t interfere much with my life. I have both a **** and a *****. Big f***ing deal.’
Lady Gaga, real name Stefani Germanotta, joked about the rumours on Twitter.
‘My show was so good last night,’ she wrote. ‘I just had to go home and suck my own hermie d***, suckka.’
Her manager’s now set the record straight, telling ABCNews.com: ‘This is completely ridiculous’.